| Molecular Mass | > 2,000-20,000 kDa
Measured by Field-Flow-Fractionation technology* |
| Biotin Binding Capacity, 50% Intercept | >350 pmol/ml
The Biotin binding capacity is measured by a competitive Biotin binding assay using Biotin-HRP-conjugate on a high binding polystyrene microplate coated with 20μg/ml Polystrept R. The Biotin-HRP will be competitively replaced by Biotin. Unbound Biotin-HRP of a dilution row is measured by colorimetric spectrometry in O.D. using HRP-reaction with TMB (evaluation of
50% Intercept). |
| Concentration | >2.5mg/ml
The protein concentration is measured by spectral-photometry
(OD 280 nm-OD 402 nm). The extinction coefficient defines as follows: A280nm [ml/mg, 1cm,] =2.741. |
| Form | Turbid Solution in 0.05M PBS with 0.05% NaN3, pH 7.4
Due to the loss of substance, a filtration of the solution is not recommended. Increasing turbidity caused by aggregation over
time can falsify the photometric measurement |
| Storage/Transportation | 2 to 8 °C, Do not freeze!
During transportation a short term storage at temperatures
<50°C do not influence the product quality negative. |
| Handling | Polystrept R is a coating solution concentrate, dilutable with PBS. Avoid solutions above pH 8. |
| Usage Statement | Laboratory reagent for research use only. |
| Quality Parameter | The quality control of each batch Polystreptavidin R composes of the following tests:
1. biotin binding capacity
2. concentration
The molecular mass is confirmed by size exclusion
chromatography during the manufacturing procedure. |
